Practical Asymmetric Enzymatic Reduction through Discovery of a Dehydrogenase-Compatible Biphasic Reaction Media
Harald Gröger*, Werner Hummel, Stefan Buchholz, Karlheinz Drauz, Tien Van Nguyen, Claudia Rollmann, Hendrik Hüsken, and Kofi Abokitse
Institute of Organic Chemistry, University of Erlangen-Nuremberg,
Henkestrasse 42, 91054 Erlangen, Germany, Email: harald.groeger
chemie.uni-erlangen.de
H. Groeger, W. Hummel, S. Buchholz, K. Drauz, T. V. Nguyen, C. Rollmann, H. Huesken, K. Abokitse, Org. Lett., 2003, 5, 173-176.
DOI: 10.1021/ol0272139
Abstract
In a biphasic reaction media for the asymmetric biocatalytic reduction of ketones with in situ cofactor regeneration, both enzymes (ADH and FDH) remain stable. Reductions with poorly water-soluble ketones were carried out at substrate concentrations of > 10 mM, and alcohols were formed with good conversions in high enantioselectivity.
see article for more examples
concept of enzymatic reduction in biphasic media
Key Words
enzymes, alcohol dehydrogenase, reduction of carbonyl compounds, formic acid
ID: J54-Y2003-270
