Categories: Analytical Chemistry >> MS, NMR

LC/MS: A Practical User's Guide

Description

Presents technical data in an easily understood format, explains how to keep LC/MS systems up and running, and shows how to interpret and quantitate the mass spectral data using proven spectral databases, without devolving into a university-style course curriculum.

• Takes a purely practical approach to using and maintaining LC/MS systems
• Introduces the reader to quadrupole and ion trap analyzers, which make up the bulk of the equipment being sold for laboratory and analytical studies
• Presents technical data in an illustrated, easily understood format
• Explains how to keep systems up and running
• Shows how to interpret and quantitate mass spectral data using proven spectral databases

Editorial Review

Over a relatively short period, LC/MS has developed into a reliable tool when applied to the rapid analysis of substance mixtures. As a professional system for reaction process control, LS/MS is nearly indispensable these days. Thus, the number of by-products is evident within a few minutes from the chromatogram, and the molecular mass data yields early indications regarding the progress and selectivity of the reaction. The number of instrumentation systems purchased for industrial labs speaks for itself. An overview on the topic of LC/MC such as the present one has been long awaited. Particularly for those industrial and academic research laboratories where non-specialists have access to the instrumentation, having a few usage guidelines can be a welcome change.

The author has parlayed his many years of experience in research and industry into a very readable book, which is primarily aimed at practitioners. Information on machine design, the overview of column materials, the features of various columns and specific application notes are all helpful in making it possible for the LC/MS and associated equipment to produce results that are reliable and reproducible. Pointers on solvents and the optimization of separations are never in short supply, as are cautions regarding the stumbling blocks with which the author has become familiar first-hand. Readers will benefit from the extensive experience captured in this work, and will be able to avoid many common pitfalls.

This book will be a very worthwhile investment for companies that operate LC/MS systems, since it will help to keep service calls on these instruments to a minimum. Students who have an interest in analytical work should also take a look at this book for use as a supplement to works on the subject of structure elucidation. Nowhere else can one find a monograph that provides such a clear explanation of HPLC and MS techniques and the fundamentals of separating substances by HPLC.

Contents

Introduction to LC/MS.
The HPLC System.
The HPLC Column and Separation Modes.
HPLC and Column Maintenance.
Sample Preparation and Separations Development.
LC/MS Interfaces.
LC/MS Overview.
The Mass Analyzers.
Mass Spectrometer Maintence.
Application Areas for LC/MS.
Trace Analysis and LC/MS/MS.
Drug Discovery and Benchtop LC/MS.
Proteomics: LC/MALDI/TOF and MS/MS Libraries.
The Future of LC/MS.