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Categories: Organic Chemistry >> Bio >> Biocatalysis

Modern Biocatalysis: Stereoselective and Environmentally Friendly Reactions

Wolf-Dieter Fessner, Thorleif Anthonsen

Hardcover, 400 Pages
First Edition, 2009
ISBN: 978-3-527-32071-4


This reference covers the wide and rapidly growing field of biocatalysis. It combines complementary expertise from such areas as microbiology, enzymology, molecular biology structural biology and organic chemistry, thus highlighting the interdisciplinary nature of the subject.

With its special focus on progress and new developments towards environmentally beneficial reactions with high levels of selectivity for the production of key compound classes, this book will enlighten both chemists and biologists as to the advances and opportunities existing in enzyme catalysis.

Editorial Review

Biocatalysis is fascinating field that continues to evolve, occasionally producing surprises such as biocatalyzed Baeyer-Villiger reactions. To give an overview of all enzyme-catalyzed reactions would definitely be the wrong way to go, since such a systematic collection presumably could not be rendered in a usable format. Therefore, the editors of this monograph have taken the right approach in limiting themselves to important and particularly interesting enzyme-catalyzed reactions, and moreover to focus on industrial applications, for example, such as employing alternative solvents or the use of microreactors.

The make-up of the chapters is a bit surprising and very unsystematic, so that there are chapters with basic techniques such as fluorescence assays for screening enzyme libraries and immobilization mixed together with specific applications. With the scope and depth of the texts, however, the reader will happy discover that the majority of the authors made a significant effort with the introductions and the number of examples, whereas three of the chapters rather limit themselves more to a brief overview. A few of the later portions certainly appear to be quite specific, making the book appear more as a collection of good review articles that can be read through when the need arises, rather than as a textbook to be worked through linearly. It is quite otherwise for the initial chapters, however, which explain fundamental techniques and are absolutely worth reading.

The book is thus to be recommended to anyone who is interested in strengthening their knowledge of enzyme-catalyzed reactions, and specializing in industrial applications. Researchers who do scale-up work in industry will find some interesting techniques and important reactions described, so that getting hold of this book will be worthwhile..


1. Fluorescence Assays for Biotransformation (Jean-Louis Reymond).
2. Immobilization as a Tool for Improving Enzymes (Ulf Hanefeld).
3. Continuous-flow Microchannel Reactors with Surface-Immobilized biocatalysts (Malene S. Thomsen and Bernd Nidetzky).
4. Activity and Stability of Proteases in Hydrophilic Solvents (Lars Haastrup Pedersen, Sinthuwat Ritthitham, and Morten Kristensen).
5. Importance of Enzyme Formulation for the Activity and Enantioselectivity of Lipases in Organic Solvents (Francesco Secundo).
6. Direct Esterification with Dry Mycelia of Molds: a (Stereo) selective, Mild and Efficient Method for Obtaining Structurally Diverse Esters (Francesco Molinari, Diego Romano, Raffaella Gandolfi, Lucia Gardossi, Ulf Hanefeld, Attilio Converti and Patrizia Spizzo).
7. Factors Affecting Enantioselectivity: Allosteric Effects (Elisabeth Egholm Jacobsen and Thorlief Anthonsen).
8. Kinetic Resolution of Sec-alcohol in Non-conventional Media (Maja Habulin, Mateja Primozic and Zeljko Knez).
9. Strategies for the biocatalyst Lipophilization of Phenolic Antioxidants (Maria H. Katsoura, Eleni Theodosiou, Haralambos Stamatis and Fragiskos N. Kolisis).
10. Biocatalysis Applied to the Synthesis of Nucleoside Analogs (Vincente Gotor).
11. Efficient Fructooligosaccharide Synthesis with a Fructosyltransferase from Aspergillus aculeatus (Francisco J. Plou,Miguel Alcalde, Iraj Ghazi, Lucia Fernandez-Arrojo and Antonio Ballesteros).
12. Hydantoin Racemase: the Key Enzyme for the Production of Optically Pure x-Amino Acids (Francisco Javier Las Heras-Vazquez, Josefa Maria Clemente-Jimenez, Sergio Martinex-Rodriguez and Felipe Rodriguez-Vico).
13. Chemo-enzymatic Deracemization Methods (Davide Tessaro, Gianluca Molla, Lorendano Pollegioni and Stefano Servi).
14. Nitrilases from Filamentous Fungi (Ludmila Martinkova, Vojtech Vejvoda, Ondrej Kaplan, Vladimir Kren, Karel Bezouska and Maria Cantarella).
15. Nitrilase- and Nitrile Hydratase-catalyzed Enantioselective Preparation of Non-proteinogenic Amino Acids (Morbert Klempier and Margit Winkler).
16. Nitrilases in the Enantioselective Synthesis of x-Hydroxycarboxylic Acids (Fred van Rantwijk, Cesar Mateo, Andrzej Chmura, Bruno C. M. Fernandes and Roger A. Sheldon).
17. UF-Membrane bioreactors for Kinetics Characterization of Nitrile Hydratase-Amidase-catalyzed reactions: a Short Survey (Maria Cantarella, Alberto Gallifuoco, Agata Spera, Laura Cantarella, Ondrej Kaplan and Ludmila Martinkova).
18. Enzymes Catalyzing C-C bond Formation for the Synthesis of Monosaccharide Analogs (Laurence Hecquet, Virgil Helaine, Franck Charmantray and Marielle Lemaire).
19. Novel Strategies in Aldolase-catalyzed Synthesis of Iminosugars (Pere Clapes, Georg A. Sprenger and Jesus Joglar).
20. Biocatalytic Asymmetric Oxidations with Oxygen (Roland Wohlgemuth).
21. Second Generation Baeyer-Villiger Biocatalysts (Veronique Alphand, Marco W. Fraaije, Marko D. Mihovilovic and Gianluca Ottolina).